High-resolution phylogenetic microbial community profiling

@inproceedings{Singer2016HighresolutionPM,
  title={High-resolution phylogenetic microbial community profiling},
  author={Esther Singer and Brian Bushnell and Devin Coleman-Derr and Brett Allen Bowman and Robert Michael Bowers and Asaf Levy and Esther Anna Gies and Jan-fang Cheng and Alex Copeland and Hans-Peter Klenk and Steven J. Hallam and Philip Hugenholtz and Susannah G Tringe and Tanja Woyke},
  booktitle={The ISME Journal},
  year={2016}
}
Over the past decade, high-throughput short-read 16S rRNA gene amplicon sequencing has eclipsed clone-dependent long-read Sanger sequencing for microbial community profiling. The transition to new technologies has provided more quantitative information at the expense of taxonomic resolution with implications for inferring metabolic traits in various ecosystems. We applied single-molecule real-time sequencing for microbial community profiling, generating full-length 16S rRNA gene sequences at… CONTINUE READING
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