High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

@article{Inomata2009HighresolutionMN,
  title={High-resolution multi-dimensional NMR spectroscopy of proteins in human cells},
  author={Kohsuke Inomata and Ayako Ohno and Hidehito Tochio and Shin Isogai and Takeshi Tenno and Ikuhiko Nakase and Toshihide Takeuchi and Shiroh Futaki and Yutaka Ito and Hidekazu Hiroaki and Masahiro Shirakawa},
  journal={Nature},
  year={2009},
  volume={458},
  pages={106-109}
}
In-cell NMR is an isotope-aided multi-dimensional NMR technique that enables observations of conformations and functions of proteins in living cells at the atomic level. This method has been successfully applied to proteins overexpressed in bacteria, providing information on protein–ligand interactions and conformations. However, the application of in-cell NMR to eukaryotic cells has been limited to Xenopus laevis oocytes. Wider application of the technique is hampered by inefficient delivery… CONTINUE READING
BETA

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 118 CITATIONS

FILTER CITATIONS BY YEAR

2009
2019

CITATION STATISTICS

  • 7 Highly Influenced Citations

  • Averaged 9 Citations per year over the last 3 years

References

Publications referenced by this paper.
SHOWING 1-10 OF 33 REFERENCES

SPARKY 3 (University of California, 1999). doi:10.1038/nature07839 Macmillan Publishers Limited

  • T. D. Goddard, D. G. Kneller
  • 2009

In situ observation of protein phosphorylation by high-resolution NMR spectroscopy

  • P Selenko
  • Nature Struct. Mol. Biol
  • 2008

The design of guanidinium-rich transporters and their internalization mechanisms

  • Wender, A P.
  • Adv. Drug Deliv. Rev
  • 2008

Similar Papers

Loading similar papers…