High resolution melting applications for clinical laboratory medicine.

@article{Erali2008HighRM,
  title={High resolution melting applications for clinical laboratory medicine.},
  author={Maria Erali and Karl V. Voelkerding and Carl T. Wittwer},
  journal={Experimental and molecular pathology},
  year={2008},
  volume={85 1},
  pages={50-8}
}
Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA that is conveniently monitored with fluorescence. Conventional melting is performed after PCR on any real-time instrument to monitor product purity (dsDNA dyes) and sequence (hybridization probes). Recent advances include high resolution instruments and saturating DNA dyes that distinguish many different species. For example, mutation scanning (identifying heterozygotes) by melting is closed-tube and has… CONTINUE READING