High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer.

@article{Simi2008HighresolutionMA,
  title={High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer.},
  author={Lisa Simi and Nicola Pratesi and Marina Vignoli and Roberta Sestini and F. Cianchi and Rosa Valanzano and Stefania Nobili and Enrico Mini and Mario Pazzagli and C. James Orlando},
  journal={American journal of clinical pathology},
  year={2008},
  volume={130 2},
  pages={247-53}
}
High-resolution melting analysis (HRMA) provides a valid approach to efficiently detect DNA genetic and somatic mutations. In this study, HRMA was used for the screening of 116 colorectal cancers (CRCs) to detect hot-spot mutations in the KRAS and BRAF oncogenes. Mutational hot spots on the PIK3CA gene, exons 9 and 20, were also screened. Direct sequencing was used to confirm and characterize HRMA results. HRMA revealed abnormal melting profiles in 65 CRCs (56.0%), 16 of them harboring… CONTINUE READING
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