High-resolution mass spectrometry of small molecules bound to membrane proteins


Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

DOI: 10.1038/nmeth.3771

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@inproceedings{Gault2016HighresolutionMS, title={High-resolution mass spectrometry of small molecules bound to membrane proteins}, author={Joseph Gault and Joseph A C Donlan and Idlir Liko and Jonathan T S Hopper and Kallol Gupta and Nicholas G. Housden and Weston B. Struwe and Michael T Marty and Todd H Mize and Ch{\'e}rine Bechara and Ya Xian Zhu and Beili Wu and Colin Kleanthous and Mikhail E. Belov and Eugen N Damoc and Alexander A. Makarov and Carol V Robinson}, booktitle={Nature methods}, year={2016} }