High-resolution mapping of transcription factor binding sites on native chromatin

@inproceedings{Kasinathan2013HighresolutionMO,
  title={High-resolution mapping of transcription factor binding sites on native chromatin},
  author={Sivakanthan Kasinathan and Steven Henikoff},
  booktitle={Epigenetics & Chromatin},
  year={2013}
}
  • Sivakanthan Kasinathan, Steven Henikoff
  • Published in Epigenetics & Chromatin 2013
  • Biology
  • Sequence-specific DNA-binding proteins including transcription factors (TFs) are key determinants of gene regulation and chromatin architecture. Formaldehyde cross-linking and sonication followed by Chromatin ImmunoPrecipitation (X-ChIP) is widely used for profiling of TF binding, but is limited by low resolution and poor specificity and sensitivity. We present a simple protocol that starts with micrococcal nuclease-digested uncross-linked chromatin and is followed by affinity purification of… CONTINUE READING

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