High-resolution isotope-dilution mass spectrometry using metabolism of isotope-labeled compounds: application to drug metabolites.

@article{Vrbanac2012HighresolutionIM,
  title={High-resolution isotope-dilution mass spectrometry using metabolism of isotope-labeled compounds: application to drug metabolites.},
  author={J James Vrbanac and Allen Hilgers and Tyson Dubnicka and F Barclay Shilliday and David Humphries and Roger N. Hayes},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2012},
  volume={26 22},
  pages={2569-76}
}
RATIONALE Herein we describe a generic quantitative method using high-resolution, isotope-dilution (HRID) metabolism of isotope-labeled compounds and apply it to the analysis of drug metabolites (DMs) in human plasma. Metabolites (drug) in Safety Testing (MIST) application was one goal. METHODS Testosterone (T) and diclofenac (D) were chosen for mass defect characteristics. T, [(14)C]T, [(13)C(3)]T, D, [(14)C]D, and [(13)C(6)]D were metabolized separately in vitro to produce test metabolites… CONTINUE READING
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