High-resolution in situ hybridization using DNA halo preparations.

  title={High-resolution in situ hybridization using DNA halo preparations.},
  author={Joop C. A. G. Wiegant and Wouter Kalle and L. H. Mullenders and Sharon M. Brookes and Jan M. N. Hoovers and Johannnes G Dauwerse and Gertjan JB van Ommen and Anton K. Raap},
  journal={Human molecular genetics},
  volume={1 8},
To improve DNA resolution of fluorescence in situ hybridization we have adapted a nuclear extraction technique, resulting in highly extended DNA loops arranged around the nuclear matrix in a halo-like structure. In situ hybridization signals from alphoid and cosmid DNAs appear as beads-on-a-string, which, according to preliminary experiments, results from the association of individual probe fragments. By multicolor hybridizations we have been able to determine relative map position and to… CONTINUE READING
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