High resolution footprinting of the hepatitis C virus polymerase NS5B in complex with RNA.

@article{Deval2007HighRF,
  title={High resolution footprinting of the hepatitis C virus polymerase NS5B in complex with RNA.},
  author={Jerome Deval and Claudia M. D’Abramo and Zhuojun Zhao and Suzanne Mccormick and Dimitrios Coutsinos and Sonja Hess and Mamuka Kvaratskhelia and Matthias G{\"o}tte},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 23},
  pages={
          16907-16
        }
}
The nucleic acid binding channel of the hepatitis C virus RNA polymerase remains to be defined. Here we employed complementary footprinting techniques and show that the enzyme binds to a newly synthesized duplex of approximately seven to eight base pairs. Comparative analysis of surface topologies of free enzyme versus the nucleoprotein complex revealed certain lysines and arginines that are protected from chemical modification upon RNA binding. The protection pattern helps to define the… CONTINUE READING

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