High resolution dual laser flow cytometry.

  title={High resolution dual laser flow cytometry.},
  author={Phillip N. Dean and Daniel Pinkel},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  volume={26 8},
Flow cytometers based on optical sensing utilize external light sources and fluorescent dyes to measure one or more specific components or properties of individual cells or subcellular particles in liquid suspension. To provide for independent excitation of two dyes used in double staining experiments we have constructed a high resolution flow cytometer that uses two laser beams to provide two wavelengths of excitation. These beams are separated spatially so that cells flow through them… CONTINUE READING