High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability.

@article{EisenbergDomovich2005HighresolutionCS,
  title={High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability.},
  author={Yael Eisenberg-Domovich and Vesa P. Hyt{\"o}nen and Meir Wilchek and Edward A Bayer and Markku S. Kulomaa and Oded Livnah},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2005},
  volume={61 Pt 5},
  pages={528-38}
}
The chicken avidin gene belongs to an extended gene family encoding seven avidin-related genes (AVRs), of which only avidin is expressed in the chicken. The sequences of AVR4 and AVR5 are identical and the common protein (AVR4) has been expressed both in insect and bacterial systems. The recombinant proteins are similarly hyperthermostable and bind biotin… CONTINUE READING