High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism.

@article{Lunin2004HighresolutionCS,
  title={High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism.},
  author={Vladimir V. Lunin and Yunge Li and Robert J. Linhardt and Hirofumi Miyazono and Mamoru Kyogashima and Takuji Kaneko and Alexander W. Bell and Miroslaw Cygler},
  journal={Journal of molecular biology},
  year={2004},
  volume={337 2},
  pages={367-86}
}
Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate but not on dermatan sulfate. Like other chondroitin AC lyases, it is capable of cleaving hyaluronan. We have determined the three-dimensional crystal structure of ArthroAC in its native form as well as in complex with its substrates (chondroitin 4-sulfate… CONTINUE READING