High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.

@article{Zabel1983HighresolutionCL,
  title={High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.},
  author={Bernard Zabel and S. L. Naylor and Alan Y. Sakaguchi and Graeme I. Bell and T. B. Shows},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 22},
  pages={6932-6}
}
The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism… CONTINUE READING

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