High resolution analysis of functional determinants on human tissue-type plasminogen activator.

@article{Bennett1991HighRA,
  title={High resolution analysis of functional determinants on human tissue-type plasminogen activator.},
  author={William F. Bennett and Nicholas F Paoni and Bruce A. Keyt and David Botstein and Andrew J. S. Jones and Leonard G. Presta and F. M. Wurm and Mark J. Zoller},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 8},
  pages={5191-201}
}
Sixty-four variants of human tissue-type plasminogen activator (tPA) were produced using recombinant DNA techniques. Charged residues were converted to alanine in clusters of from one to four changes per variant; these clusters spanned all the domains of the molecule. The variants were expressed by mammalian cells and were analyzed for a variety of properties. Variants of tPA were found that had reduced activity with respect to each tested property; in a few cases increased activity was… CONTINUE READING

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