High-resolution NMR studies of encapsulated proteins in liquid ethane.

  title={High-resolution NMR studies of encapsulated proteins in liquid ethane.},
  author={Ronald W Peterson and Brian G Lefebvre and A Joshua Wand},
  journal={Journal of the American Chemical Society},
  volume={127 29},
Many of the difficulties presented by large, aggregation-prone, and membrane proteins to modern solution NMR spectroscopy can be alleviated by actively seeking to increase the effective rate of molecular reorientation. An emerging approach involves encapsulating the protein of interest within the protective shell of a reverse micelle and dissolving the resulting particle in a low viscosity fluid, such as the short chain alkanes. Here we present the encapsulation of proteins with high structural… CONTINUE READING