High-resolution N(6) -methyladenosine (m(6) A) map using photo-crosslinking-assisted m(6) A sequencing.

  title={High-resolution N(6) -methyladenosine (m(6) A) map using photo-crosslinking-assisted m(6) A sequencing.},
  author={Kai Chen and Zhike Lu and Xiao Wang and Ye Fu and Guanzheng Luo and N Liu and Dali Han and Dan Dominissini and Qing Dai and Tao Pan and Chuan He},
  journal={Angewandte Chemie},
  volume={54 5},
N(6) -methyladenosine (m(6) A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m(6) A modification remain limited. We present here a photo-crosslinking-assisted m(6) A sequencing strategy (PA-m(6) A-seq) to more accurately define sites with m(6) A modification. Using this strategy, we obtained a high-resolution map of m(6) A in a human transcriptome. The map resembles the general distribution pattern… CONTINUE READING

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