High-resolution DNA melting analysis for simple and efficient molecular diagnostics.

  title={High-resolution DNA melting analysis for simple and efficient molecular diagnostics.},
  author={Gudrun H Reed and Jana O Kent and Carl T. Wittwer},
  volume={8 6},
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and sequence matching. The melting profile of a PCR product depends on its GC content, length, sequence and heterozygosity and is best monitored with saturating dyes that fluoresce in the presence of double-stranded DNA. Genotyping of most variants is possible by the melting temperature of the PCR products, while all variants can be genotyped with unlabeled probes. Mutation scanning and sequence matching… CONTINUE READING
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