High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.

@article{Milhiet2006HighresolutionAO,
  title={High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.},
  author={Pierre-Emmanuel Milhiet and Francesca Gubellini and Alexandre Berquand and Patrice Dosset and J. Rigaud and C Christian Le Grimellec and Daniel L{\'e}vy},
  journal={Biophysical journal},
  year={2006},
  volume={91 9},
  pages={3268-75}
}
The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct incorporation of solubilized proteins into a preformed planar lipid bilayer destabilized by dodecyl-beta-maltoside or dodecyl-beta-thiomaltoside, two detergents widely used in… CONTINUE READING

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