High resolution 31P nuclear magnetic resonance studies of intact yeast cells.

@article{Salhany1975HighR3,
  title={High resolution 31P nuclear magnetic resonance studies of intact yeast cells.},
  author={J. Mitchell Salhany and Takashi Yamane and Robert G. Shulman and Seiichiro Ogawa},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1975},
  volume={72 12},
  pages={
          4966-70
        }
}
High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our results are consistent with the suggestion that these polyphosphates act as a phosphate store in the cell. We have also been able to measure cytoplasmic pH using the orthophosphate peak inside the cell, as compared with outside the cell. The results show that… CONTINUE READING

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