High quality RNA extraction of the mammalian cochlea for qRT-PCR and transcriptome analyses.


Molecular investigations of the hearing organ, the cochlea, have been hampered due to the difficulty of isolating pure RNA and in quantities sufficient enough for quantitative real-time RT-PCR or microarray analysis. The complex architecture of the cochlea, the presence of liquids, bone and cartilage tissue, are a major hurdle in obtaining contamination… (More)
DOI: 10.1016/j.heares.2015.03.008