High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.

@article{Bowers1980Highpurity4P,
  title={High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.},
  author={G. Bowers and R. Mccomb and R. G. Christensen and R. Schaffer},
  journal={Clinical chemistry},
  year={1980},
  volume={26 6},
  pages={
          724-9
        }
}
We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper molar absorptivity of 4-nitrophenol under final reaction conditions, particularly for measuring alkaline phosphatase activity in human serum. Some lots of 4-nitrophenol available commercially met these specifications, but several did not. The latter can be purified to meet our specifications by… Expand
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