High production of laccase by a new basidiomycete, Trametes sp

@article{Tong2006HighPO,
  title={High production of laccase by a new basidiomycete, Trametes sp},
  author={Pingui Tong and Yuzhi Hong and Yazhong Xiao and Min Zhang and Xiaoming Tu and Tengjiao Cui},
  journal={Biotechnology Letters},
  year={2006},
  volume={29},
  pages={295-301}
}
A new basidiomycete, Trametes sp. 420, produced laccase at 6,810 U l−1 (268 mg, 25.4 U mg−1 protein for guaiacol) in glucose medium and 7,870 U l−1 (310 mg) in cellobiose medium with induction by 0.5 mM Cu2+ and 6 mM o-toluidine. Laccase isozyme E (LacE) was the sole laccase in the fermentation products. It was stable at pH 5–9 and below 70°C over 30 min. The K m values of LacE for four substrates (guaiacol ABTS, 2,6-dimethoxyphenol and syringaldazine) varied from 5 to 245 μM. The activity of… CONTINUE READING