High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system

@article{SaintAmans2004HighPO,
  title={High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system},
  author={Sylvie Saint-Amans and Patrice Perlot and Gerard Goma and Philippe Soucaille},
  journal={Biotechnology Letters},
  year={2004},
  volume={16},
  pages={831-836}
}
SummaryA simple fed-batch system which controls substrate feeding by measuring the CO2 produced during the fermentation, was developped. This Fed-batch approach allowed high production of 1,3-propanediol from glycerol by Clostridium butyricum by avoiding substrate inhibition phenomena. 65 g/l of 1,3-propanediol was produced with a productivity of 1.21 g/l.h and a yield of 0.56. The concentration of 1,3-propanediol obtained and the productivity were significantly higher than those reached in… 
Production of 1,3-propanediol by Clostridium butyricum DSM 5431 and product tolerant mutants in fedbatch culture: Feeding strategy for glycerol and ammonium
TLDR
A fedbatch strategy was developed coupling the feeding of the two inhibitory substrates glycerol and ammonium to alkali consumption responding directly to the needs of the culture, resulting in considerably shortened cultivation times.
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
TLDR
A high and constant 1,3-propanediol yield, close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate, and 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.
Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process
TLDR
Investigation of the production of 1,3-propanediol (PDO) under non-sterile fermentation conditions by employing the strain Clostridium butyricum VPI 1718 revealed the stability of the bioprocess proposed, with global PDO production yield corresponding to 0.52 g/g.
Production of 1,3-propanediol by Clostridium butyricum VPI 3266 using a synthetic medium and raw glycerol
TLDR
1,3-propanediol production from two raw glycerol types, without any prior purification, was observed in batch and continuous cultures, on a synthetic medium, and no significant differences were found in C. butyricum fermentation patterns on raw and commercial Glycerol as the sole carbon source.
1,3-Propanediol Production by Clostridium Butyricum in Various Fed-Batch Feeding Strategies
TLDR
Different fed-batch strategies during the fermentation of glycerol into 1,3-Propanediol are proposed, which show the influence of the initial concentration in Glycerol and the feed flow on the behaviour of Clostridium butyricum.
Scale-up of anaerobic 1,3-propanediol production by Clostridium butyricum DSP1 from crude glycerol
TLDR
The analysis of cell proteins demonstrated that a number of multifunctional stresses occurred during fed-batch fermentations in the 150 L bioreactor, which suggests the possibility of identifying the key stages in the biochemical process where inhibition of 1,3-PD synthesis pathways can be observed.
Simple Strategy of Repeated Batch Cultivation for Enhanced Production of 1,3-Propanediol Using Clostridium diolis
TLDR
This is the first report on the use of repeated batch mode of bioreactor operation for enhanced 1,3-PD production and productivity enhancement by 2.6-fold and 1.5-fold, respectively, as compared to batch 1, 3-PD fermentation.
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