High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system

  title={High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system},
  author={Sylvie Saint-Amans and Patrice Perlot and Gerard Goma and Philippe Soucaille},
  journal={Biotechnology Letters},
SummaryA simple fed-batch system which controls substrate feeding by measuring the CO2 produced during the fermentation, was developped. This Fed-batch approach allowed high production of 1,3-propanediol from glycerol by Clostridium butyricum by avoiding substrate inhibition phenomena. 65 g/l of 1,3-propanediol was produced with a productivity of 1.21 g/l.h and a yield of 0.56. The concentration of 1,3-propanediol obtained and the productivity were significantly higher than those reached in… 
Production of 1,3-propanediol by Clostridium butyricum DSM 5431 and product tolerant mutants in fedbatch culture: Feeding strategy for glycerol and ammonium
A fedbatch strategy was developed coupling the feeding of the two inhibitory substrates glycerol and ammonium to alkali consumption responding directly to the needs of the culture, resulting in considerably shortened cultivation times.
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
A high and constant 1,3-propanediol yield, close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate, and 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.
Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process
Investigation of the production of 1,3-propanediol (PDO) under non-sterile fermentation conditions by employing the strain Clostridium butyricum VPI 1718 revealed the stability of the bioprocess proposed, with global PDO production yield corresponding to 0.52 g/g.
Production of 1,3-propanediol by Clostridium butyricum VPI 3266 using a synthetic medium and raw glycerol
1,3-propanediol production from two raw glycerol types, without any prior purification, was observed in batch and continuous cultures, on a synthetic medium, and no significant differences were found in C. butyricum fermentation patterns on raw and commercial Glycerol as the sole carbon source.
1,3-Propanediol Production by Clostridium Butyricum in Various Fed-Batch Feeding Strategies
Different fed-batch strategies during the fermentation of glycerol into 1,3-Propanediol are proposed, which show the influence of the initial concentration in Glycerol and the feed flow on the behaviour of Clostridium butyricum.
Scale-up of anaerobic 1,3-propanediol production by Clostridium butyricum DSP1 from crude glycerol
The analysis of cell proteins demonstrated that a number of multifunctional stresses occurred during fed-batch fermentations in the 150 L bioreactor, which suggests the possibility of identifying the key stages in the biochemical process where inhibition of 1,3-PD synthesis pathways can be observed.
Simple Strategy of Repeated Batch Cultivation for Enhanced Production of 1,3-Propanediol Using Clostridium diolis
This is the first report on the use of repeated batch mode of bioreactor operation for enhanced 1,3-PD production and productivity enhancement by 2.6-fold and 1.5-fold, respectively, as compared to batch 1, 3-PD fermentation.


Production of 1,3-Propanediol from Glycerol by Clostridium acetobutylicum and Other Clostridium Species
  • C. Forsberg
  • Chemistry, Biology
    Applied and environmental microbiology
  • 1987
Glycerol was fermented with the production of 1,3-propanediol as the major fermentation product by four strains of Clostridium acetobutylicum and three of C. butyricum, and 1, 3-Propanediol was identified by its retention times in gas chromatography and high-pressure liquid chromatographyand by its mass spectrum.
Glycerol conversion to 1,3-propanediol by newly isolated clostridia
The observations point to a substantial transfer of reducing potential from ferredoxin to NAD, which finally results in increased 1,3-propanediol production.
Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum up to a scale of 2m3
SummaryThe conversion of glycerol to 1,3-propanediol (PD) by Clostridium butyricum DSM 5431 was studied in anaerobic culture. Growth and product formation were optimal at pH = 7.0 and T = 35° C,
Glycerol fermentation of 1,3-propanediol by Clostridium butyricum. Measurement of product inhibition by use of a pH-auxostat
  • H. Biebl
  • Biology, Materials Science
    Applied Microbiology and Biotechnology
  • 2004
1,3-propanediol is the first range inhibitor in this fermentation, and may approach the value of complete inhibition while the concentrations of acetic and butyric acids remained below these values by at least one order of magnitude.
Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains
Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol, and Enterobacterial strains of the genera Enterobacter, Klebsiella, and Citrobacter showed similar product patterns except for one group which formed limited amounts of ethanol, but no propanediol.
The fermentation of glycerol byClostridium butyricum LMG 1212t2 and 1213t1 andC. pasteurianum LMG 3285
The data indicated the existance of a specific 1,3-propanediol dehydrogenase in both organisms and this fermentation pattern was hardly affected by acetate as co-substrate.
Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol
The metabolism of Clostridium acetobutylicum was manipulated, at neutral pH and in chemostat culture, by changing the overall degree of reduction of the substrate, using mixtures of glucose and glycerol to indicate the absence of butyraldehyde dehydrogenase activity and very low levels of coenzyme A-transferase, butanol, and ethanol dehydrogenases.
Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and diol dehydratases
Data and the stimulation of growth by Co2+ suggested that glycerol dehydratase and trimethylene glycol dehydrogenase are obligatory enzymes for anaerobic growth on Glycerol as the sole carbon source.
A gas meter for low rates of gas flow: Application to the methane fermentation
SummaryA device for measuring low rates of gas flow is described and an example of application to the methane fermentation is reported. Its principle is based on the total counts of impulsions