High prevalence of natural antibodies against Plasmodium falciparum 83-kilodalton apical membrane antigen (PF83/AMA-1) as detected by capture-enzyme-linked immunosorbent assay using full-length baculovirus recombinant PF83/AMA-1.

@article{Thomas1994HighPO,
  title={High prevalence of natural antibodies against Plasmodium falciparum 83-kilodalton apical membrane antigen (PF83/AMA-1) as detected by capture-enzyme-linked immunosorbent assay using full-length baculovirus recombinant PF83/AMA-1.},
  author={A. W. Thomas and J. F. Trape and C. Rogier and A. Goncalves and V. D. Ros{\'a}rio and D. Narum},
  journal={The American journal of tropical medicine and hygiene},
  year={1994},
  volume={51 6},
  pages={
          730-40
        }
}
  • A. W. Thomas, J. F. Trape, +3 authors D. Narum
  • Published 1994
  • Biology, Medicine
  • The American journal of tropical medicine and hygiene
  • The 83-kilodalton (kD) apical membrane antigen of Plasmodium falciparum (PF83/AMA-1) is a potential asexual blood stage vaccine component. This antigen has been expressed as a full-length, nonfusion, recombinant baculovirus protein (PF83-7G8-1) using the authentic predicted signal peptide for appropriate postsynthetic routing. When purified by a novel high-performance, ion exchange chromatography (HPIEC) method, PF83-7G8-1 induced polyclonal antibodies in rats that immunoprecipitated both 83… CONTINUE READING
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