High prevalence of Plasmodium malariae and Plasmodium ovale in malaria patients along the Thai‐Myanmar border, as revealed by acridine orange staining and PCR‐based diagnoses

@article{Zhou1998HighPO,
  title={High prevalence of Plasmodium malariae and Plasmodium ovale in malaria patients along the Thai‐Myanmar border, as revealed by acridine orange staining and PCR‐based diagnoses},
  author={M. Zhou and Q. Liu and Chansuda Wongsrichanalai and W Suwonkerd and Kamolwan Panart and Somsak Prajakwong and A Pensiri and Masatsugu Kimura and Hiroyuki Matsuoka and M U Ferreira and Shin Isomura and Fumihiko Kawamoto},
  journal={Tropical Medicine \& International Health},
  year={1998},
  volume={3}
}
The prevalence of the four human malaria parasites was investigated among malaria patients at northern, central and southern towns in Thailand along the border with Myanmar between September 1995 and May 1996. Thin smears obtained from 548 Thai and Burmese patients were reviewed by an acridine orange staining method, and many mixed infections with two to four species, including P. malariae and P. ovale, were detected. These diagnostic results were compared with those by two PCR‐based diagnoses… Expand
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References

SHOWING 1-10 OF 26 REFERENCES
Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam
TLDR
Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis, which indicated that in addition to molecular biological methods, careful microscopic examination of stained thin blood films is still required in studies of the prevalence of different malaria species. Expand
Species-specific PCR detection of malaria parasites by microtiter plate hybridization: clinical study with malaria patients
TLDR
This PCR method is highly sensitive and specific for the detection of all four parasite species and can serve as a useful supplement to microscopy for the clinical management of malaria. Expand
A Colorimetric DNA Diagnostic Method for Falciparum Malaria and Vivax Malaria: A Field Trial in the Solomon Islands
TLDR
A colorimetric assay, “microtiter plate-hybridization”, for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood is developed, in which the target DNA sequences are hybridized with the species-specific probes immobilized on a microtiter well. Expand
Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy.
TLDR
A prospective 'blinded' comparison of diagnosis by conventional light microscopy and enzymatic amplification of the circumsporozoite gene extracted from dried spotted blood samples found P. falciparum and P. vivax infections to be mixed by the PCR/DNA probe systems. Expand
The importance of sensitive detection of malaria parasites in the human and insect hosts in epidemiological studies, as shown by the analysis of field samples from Guinea Bissau.
TLDR
A method based on the polymerase chain reaction for highly sensitive detection and identification of human malaria parasites was applied to blood and mosquito samples obtained from a village in Guinea Bissau and preliminary evidence was obtained to show that the distribution of P. malariae infections within the village was non-random. Expand
Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections.
TLDR
Genus- and species-specific sequences are present within the small subunit ribosomal RNA genes of the four human malaria parasites that have proven to be more sensitive and accurate than by routine diagnostic microscopy. Expand
Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene
Abstract A polymorphic region of the small subunit rRNA gene of the four human malaria parasite species was sequenced to see intraspecies variations. Two new variant sequences were found in P. ovaleExpand
Imported malaria in Montagnard refugees settling in North Carolina: implications for prevention and control.
TLDR
This group of Southeast Asian refugees resettled in North Carolina accounted for the largest cluster of malaria cases reported in the United States in the last 50 years and resulted in a significant burden to the refugees and to the health-care system. Expand
Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries.
TLDR
It is concluded that the QBC technique alone cannot replace Giemsa-stained thick blood films for most purposes in an African setting, but apart from species differentiation, the AO method is an appropriate technique for the laboratory diagnosis of malaria in developing countries. Expand
DNA diagnosis of ovale malaria and malariae malaria using microtiter plate-hybridization
Abstract We have developed a colorimetric assay, “microtiter plate-hybridization”, for the detection of human malaria parasite, Plasmodium ovale and Plasmodium malariae, in which the target DNAExpand
...
1
2
3
...