High-pressure treatment of polytene chromosomes improves structural resolution

@article{Novikov2007HighpressureTO,
  title={High-pressure treatment of polytene chromosomes improves structural resolution},
  author={Dmitry V. Novikov and Igor Kireev and Andrew S. Belmont},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={483-485}
}
The exceptional cytology provided by polytene chromosomes has made Drosophila melanogaster a premier model for chromosome studies, but full exploitation of polytene cytology is impeded by the difficulty in preparing high-quality chromosome spreads. Here we describe use of high pressure to produce formaldehyde-fixed chromosome spreads, which upon light-microscopy examination reveal structural detail previously observed only in electron microscopy preparations. We demonstrate applications to… CONTINUE READING
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