High pressure liquid chromatography and mass spectrometry characterization of the nephrotoxic biotransformation products of Cisplatin.

@article{Townsend2003HighPL,
  title={High pressure liquid chromatography and mass spectrometry characterization of the nephrotoxic biotransformation products of Cisplatin.},
  author={Danyelle M. Townsend and Jarrod A. Marto and Mei Deng and Timothy J Macdonald and Marie H. Hanigan},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2003},
  volume={31 6},
  pages={705-13}
}
Previous studies have shown that cisplatin requires metabolic activation to become nephrotoxic. The activation is proposed to be via the metabolism of a glutathione-platinum conjugate to a cysteinyl-glycine-platinum conjugate, which is further processed to a cysteine conjugate. Preincubating cisplatin with glutathione (GSH), cysteinyl-glycine, or N-acetylcysteine (NAC) results in a transient increase in the toxicity of cisplatin toward renal proximal tubular cells. In this study, the… CONTINUE READING