Corpus ID: 37496126

High-pressure liquid chromatographic separation of 10 benzo(a)pyrene phenols and the identification of 1-phenol and 7-phenol as new metabolites.

@article{Selkirk1976HighpressureLC,
  title={High-pressure liquid chromatographic separation of 10 benzo(a)pyrene phenols and the identification of 1-phenol and 7-phenol as new metabolites.},
  author={J. Selkirk and R. G. Croy and H. Gelboin},
  journal={Cancer research},
  year={1976},
  volume={36 3},
  pages={
          922-6
        }
}
The separation of ten isomeric benzo(a)pyrene phenols has been accomplished by the use of high-pressure liquid chromatography utilizing a newly developed recycling technique and new column and solvent systems. Using this new system and comparing the metabolites obtained with authentic standards, we have isolated 1-hydroxybenzo(a)pyrene and 7-hydroxybenzo(a)pyrene and identified them as metabolites formed by rat liver microsomes. In previously reported chromatography systems, the new metabolites… Expand
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Direct fluorometric analysis of benzo(a)pyrene metabolite formation by mouse liver microsomes.
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  • Analytical biochemistry
  • 1980
TLDR
The fluorescence spectra of the total ethyl acetate-soluble metabolites clearly indicate that the metabolites produced by DBA 2 enzymes were predominantly monohydroxylated benzo( a)pyrene while those produced by the liver microsomes of C57BL 6J were highly enriched with the 7,8-dihydrodihydroxybenzo(a) pyrene type. Expand
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The epoxide metabolite has been isolated and characterized as benzo(a)pyrene-4,5-epoxide by comparison of its HPLC retention times, ultraviolet and mass spectral analysis with synthetic benzo (a) pyrene- 4,5 -epoxide and its conversion by liver microsomes to benzos(a),5-dihydrodiol. Expand
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