High-pressure liquid chromatographic quantitation of rifampin and its two major metabolites in urine and serum.

  title={High-pressure liquid chromatographic quantitation of rifampin and its two major metabolites in urine and serum.},
  author={A. Weber and K. Opheim and A. Smith and K. Wong},
  journal={Reviews of infectious diseases},
  volume={5 Suppl 3},
  • A. Weber, K. Opheim, +1 author K. Wong
  • Published 1983
  • Medicine
  • Reviews of infectious diseases
  • Rifampin and two of its major metabolites, 25-O-desacetylrifampin and 3-formylrifamycin SV, were quantitated in serum, urine, and aqueous solutions by high-pressure, reverse-phase liquid chromatography. This rapid, precise, and simple method utilizes a single-step extraction, dual-wavelength monitoring, and quantitation by comparison of the absorbance of the drug or metabolite with that of an internal standard; the wavelengths used are 337 nm and 254 nm. The degrees of precision are +/- 1.3… CONTINUE READING
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