High-pressure freezing provides insights into Weibel-Palade body biogenesis.

  title={High-pressure freezing provides insights into Weibel-Palade body biogenesis.},
  author={Helen L Zenner and Lucy M. Collinson and Gr{\'e}goire Michaux and Daniel F Cutler},
  journal={Journal of cell science},
  volume={120 Pt 12},
The Weibel-Palade bodies (WPBs) of endothelial cells play an important role in haemostasis and the initiation of inflammation, yet their biogenesis is poorly understood. Tubulation of their major content protein, von Willebrand factor (VWF), is crucial to WPB function, and so we investigated further the relationship between VWF tubule formation and WPB formation in human umbilical vein endothelial cells (HUVECs). By using high-pressure freezing and freeze substitution before electron microscopy… CONTINUE READING