High-pressure freezing and freeze substitution of Arabidopsis for electron microscopy.

Abstract

The objectives of electron microscopy ultrastructural studies are to examine cellular architecture and relate the cell's structural machinery to dynamic functional roles. This aspiration is difficult to achieve if specimens have not been adequately preserved in a "living state"; hence specimen preparation is of the utmost importance for the success of any… (More)
DOI: 10.1007/978-1-62703-580-4_25

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