High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy

@article{Reymann2008HighprecisionSA,
  title={High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy},
  author={J{\"u}rgen Reymann and David Baddeley and Manuel Gunkel and Paul Lemmer and Werner Stadter and Thibaud Jegou and Karsten Rippe and Christoph Cremer and Udo Jochen Birk},
  journal={Chromosome Research},
  year={2008},
  volume={16},
  pages={367-382}
}
Spatially modulated illumination (SMI) microscopy is a method of wide field fluorescence microscopy featuring interferometric illumination, which delivers structural information about nanoscale architecture in fluorescently labelled cells. The first prototype of the SMI microscope proved its applicability to a wide range of biological questions. For the SMI live cell imaging this system was enhanced in terms of the development of a completely new upright configuration. This so called Vertico… 
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The application of SMI microscopy to size measurements of the 7q22 gene region is presented, giving a size estimate of 105±16 nm which corresponds to an average compaction ratio of 1:324, demonstrating that the technique is robust when applied to biological samples.
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TLDR
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