High-precision distance microscopy of 3D nanostructures by a spatially modulated excitation fluorescence microscope

@inproceedings{Hausmann1997HighprecisionDM,
  title={High-precision distance microscopy of 3D nanostructures by a spatially modulated excitation fluorescence microscope},
  author={M. Hausmann and B. Schneider and J. Bradl and C. Cremer},
  booktitle={European Conference on Biomedical Optics},
  year={1997}
}
To study the 3D-organization and 3D-pathology of the genome in intact cell nuclei, precise and accurate 3D-object localizations and 3D-distance measurements of fluorescent labelled chromatin-nanostructures are required. For this purpose, a high precision fluorescence microscope setup with spatially modulated excitation (SME) has been built up combining advantages of an epifluorescence microscope with interferometric laser illumination in the direction of the optical axis and optical sectioning… Expand
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