High performance two-dimensional gel electrophoresis using a wetting agent Tergitol NP7.

@article{LaoudjChenivesse2002HighPT,
  title={High performance two-dimensional gel electrophoresis using a wetting agent Tergitol NP7.},
  author={Dalila Laoudj-Chenivesse and Philippe Marin and Ren{\'e} Bennes and Emmanuel Tronel-Peyroz and François Leterrier},
  journal={Proteomics},
  year={2002},
  volume={2 5},
  pages={481-5}
}
Two-dimensional electrophoresis is an efficient method for the analysis of a broad range of complex protein samples. Current two-dimensional gel techniques are not suited for analysis of the small amount of proteins from tissue samples in the presence of high concentration of salts. Here we describe an improved two-dimensional gel electrophoresis procedure based on the use of a nonionic wetting agent, Tergitol NP7, in rehydration solution combined with the application of a linear potential… CONTINUE READING

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