High-performance liquid chromatography with fluorometric detection for monitoring of etoposide and its cis-isomer in plasma and leukaemic cells.

@article{Liliemark1995HighperformanceLC,
  title={High-performance liquid chromatography with fluorometric detection for monitoring of etoposide and its cis-isomer in plasma and leukaemic cells.},
  author={Eva Liliemark and Birgitta Pettersson and Curt Peterson and Jan O Liliemark},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1995},
  volume={669 2},
  pages={311-7}
}
The podophyllotoxin derivative etoposide, extensively used in anticancer therapy, is highly protein-bound (95%) in plasma. It is a chiral drug and only the trans-isomer is pharmacologically active. Isomerisation to the inactive cis-lactone occurs in plasma. The cis-lactone is often present in ultrafiltrates of plasma from patients treated with etoposide, therefore it is important to separate the isomers when free etoposide concentrations are assayed. There is reason to believe that free and… CONTINUE READING