High-performance liquid chromatography of phospholipids using deuterated solvents for infrared detection.


Infrared detection of chromatographic effluents offers the advantage of direct on-line quantitation of lipid fractions. However, infrared detection imposes limitations on the solvent systems that can be used for chromatography. Methanol and water, which are essential ingredients in the mobile phase for the successful chromatography of phospholipids, do not… (More)


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