High-performance liquid chromatographic profiling of fluorescent labelled N-glycans on glycoproteins.

@article{Yuen2002HighperformanceLC,
  title={High-performance liquid chromatographic profiling of fluorescent labelled N-glycans on glycoproteins.},
  author={C. T. Yuen and C M Gee and Cynthia Farrugia Jones},
  journal={Biomedical chromatography : BMC},
  year={2002},
  volume={16 4},
  pages={247-54}
}
Monitoring protein glycosylation is becoming increasingly important as novel recombinant glycoprotein therapeutics, such as glycoprotein hormones, cytokines and clotting factors, are introduced into clinical use. In this report, we describe an HPLC strategy and an improved and simplified pre-column derivatization procedure to profile N-linked glycans obtained from a variety of commercially available glycoproteins as examples. N-Glycans were first released by peptide:N-glycosidase F and labelled… CONTINUE READING