High-performance liquid chromatographic preparation of galactosyl-hydroxylysine, a specific bone collagen marker.

  title={High-performance liquid chromatographic preparation of galactosyl-hydroxylysine, a specific bone collagen marker.},
  author={Luigi Moro and C Battista and C. Modricky and Luciana Rovis and B de Bernard and P. Traldi and Gilles Lubrano and Francesco Marotti},
  journal={Journal of chromatography},
  volume={490 2},
Galactosyl-hydroxylysine, a specific bone collagen marker, has been prepared directly from human urine samples by high-performance liquid chromatography (HPLC) on a preparative column. The compound is the didansyl derivative, as proved by HPLC and mass spectrometry under fast atom bombardment conditions. Since this compound is not commercially available, the procedure reported appears to be the simplest way to prepare it, which is necessary to measure the urinary excretion of this collagen… Expand
A rapid and simple method for quantitation of urinary hydroxylysyl glycosides, indicators of collagen turnover, using liquid chromatography/tandem mass spectrometry.
A simple liquid chromatography/tandem mass spectrometry method is proposed for measuring glycosides of hydroxylysine in urine, with no sample preparation except for a dilution step, and is proposed as a robust tool for a large-scale research investigation on collagen turnover. Expand
High predictivity of galactosyl-hydroxylysine in urine as an indicator of bone metastases from breast cancer.
We measured the urinary excretion of galactosyl-hydroxylysine (GH) and hydroxyproline in two groups of women with breast cancer, with (M+, n = 24) and without (Mo, n = 30) clinical, scintigraphic, orExpand
Urinary excretion of galactosyl-hydroxylysine is a marker of growth in children.
It is concluded that the urinary excretion of Gal-Hyl is a valid and potentially useful index of skeletal growth in children. Expand
Galactosyl Hydroxylysine and Deoxypyridinoline: A Methodological Comparison
  • P. Bettica, D. Baylink, L. Moro
  • Medicine
  • European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies
  • 1993
This review shows that the galactosyl hydroxylysine and deoxypyridinoline HPLC assays differ mainly in the need for an acid hydrolysis and a preextraction of the urine samples. Expand
Hydroxylysine containing glycoconjugates: an efficient synthesis of natural galactosylhydroxylysine (Gal-Hyl) and glucosylgalactosylhydroxylysine (Glu-Gal-Hyl) and of their (5S)-epimers
Abstract The paper reports the first chemical synthesis of α- d -glucopyranosyl-(1→2)-β- d -galactopyranosyl-5- O -hydroxylysine, a biological marker of bone resorption and of its unnatural (5 SExpand
Two-site assays of bone gla protein (osteocalcin) demonstrate immunochemical heterogeneity of the intact molecule.
A panel of monoclonal antibodies to human bone gla protein (BGP; osteocalcin) peptides that span the linear sequence of the molecule, specifically BGP 1-12 (N-terminal), BGP 15-30 (midregion), and BGP 38-49 (C- terminal) were developed, demonstrating the immunochemical heterogeneity of circulating BGP. Expand
Clinical performances of galactosyl hydroxylysine, pyridinoline, and deoxypyridinoline in postmenopausal osteoporosis.
The validity of GHYL, PYD, and DPD as markers of bone resorption as well as logistic-regression analysis of the posterior probabilities for prediction from a logistic model are confirmed. Expand
Bone-resorption markers galactosyl hydroxylysine, pyridinium crosslinks, and hydroxyproline compared.
For modest changes, DPD, GHYL, and PYD are more accurate and have a higher discrimination power than does HYP, and in the mildly increased-BR group, D PD, GHyl, andPYD showed a higher Discrimination power and accuracy than did HYP. Expand
Posttranslational Modifications of Bone Collagen Type I are Related to the Function of Rat Femoral Regions
The conclusion is that matrix composition differs among different skeletal regions according to the main function they exert, and the amounts of the same compounds in the diaphysis and the proximal metaphysis are statistically the same. Expand
CHAPTER 33 – Measurement of Biochemical Markers of Bone Resorption
This chapter reviews both the basics and some recent progress made in the development of markers of bone resorption, a process that largely relies on the activity of ostoblasts and ostoclasts to undergo continuous remodeling. Expand


High-performance liquid chromatographic analysis of urinary hydroxylysyl glycosides as indicators of collagen turnover.
Description d'une methode rapide et sensible, impliquant la conversion des groupes amines de l'hydroxylysine en derives fluorescents a l'aide du chlorure de dansyle, avant la separation parExpand
[18] Urinary metabolites of collagen
Publisher Summary The urinary excretion of hydroxyproline and hydroxylysine has been a commonly used standard for the evaluation of collagen degradation. Free urinary hydroxylysine does not appear toExpand
Determination of galactosyl hydroxylysine in urine as a means for the identification of osteoporotic women.
The aim of the work is to assess the predictive values of the method for the identification of post-menopausal osteoporotic women by measuring galactosyl hydroxylysine in urine by HPLC. Expand
Hydroxylysine glycosides: preparation and analysis by reverse phase high performance liquid chromatography.
A reverse phase HPLC system was devised for Hyl glycosides, hydroxylysine and other basic amino acids and resulted a mixture of two diastereoisomers, which form during the alkaline hydrolysis of Hyl di- and monoglycoside. Expand
Synthesis of 0-(2-0-α-d-glucopyranosyl)-β-d-galactopyranoside of optically pure δ-hydroxy-l-lysylglycine and -gd-hydroxy-l-lysylglycyl-l-glutamyl-l- aspartylglycine
Abstract The application of the levulinyl group as a temporary blocking group for the 2-OH function of galactose proved to be very efficient not only for the introduction of a β-linkage betweenExpand
[12] Dansyl chloride procedure
Publisher Summary This chapter discusses the formation and stability of dansyl chloride procedure. Dansyl chloride is used for providing fluorescent “handles” for the study of proteins. The reactionExpand
The observation of metastable transitions in a double-focussing mass spectrometer using a linked scan of the electric sector and magnetic sector fields
A detailed study has been made of the observation of metastable transitions in the first field-free region of a double-focussing mass spectrometer of Nier—Johnson geometry by scanning simultaneouslyExpand