High performance liquid chromatographic determination of methylxanthines in canine serum, gastric and pancreatic juices.

@article{Casoli1990HighPL,
  title={High performance liquid chromatographic determination of methylxanthines in canine serum, gastric and pancreatic juices.},
  author={Paolo Casoli and H. J. Verine},
  journal={Biomedical chromatography : BMC},
  year={1990},
  volume={4 5},
  pages={209-13}
}
A convenient high performance liquid chromatographic method for the determination of methylxanthines in biological samples is described. Separation was achieved by reversed phase chromatography using a mobile phase consisting of tetrahydrofuran + methanol + 0.01M potassium dihydrogen phosphate, pH 3.5 (1:20:79, v/v/v), on a 7 microns C18 column and a C18 Lichrosorb precolumn at a flow rate of 0.8 mL/min. Levels varying from 0.25-16 mg/L could be detected by UV at 280 nm. In this range, standard… CONTINUE READING