High-performance liquid chromatographic determination of acrolein as a marker for cyclophosphamide bioactivation in human liver microsomes.

@article{Bohnenstengel1997HighperformanceLC,
  title={High-performance liquid chromatographic determination of acrolein as a marker for cyclophosphamide bioactivation in human liver microsomes.},
  author={Frank Bohnenstengel and Michel F Eichelbaum and E Golbs and Heyo Klaus Kroemer},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1997},
  volume={692 1},
  pages={163-8}
}
A high-performance liquid chromatographic method for the quantification of acrolein following incubation of cyclophosphamide (CP) with human liver microsomes was developed. Based on the formation of the fluorescent derivative 7-hydroxyquinoline by condensation of acrolein with 3-aminophenol quantitation was performed without prior extraction or other sample… CONTINUE READING