High-performance liquid chromatographic determination of alpha-amino acids in pharmaceutical dosage forms using aqueous mobile phase containing copper (II) ions.

@article{Refaat2002HighperformanceLC,
  title={High-performance liquid chromatographic determination of alpha-amino acids in pharmaceutical dosage forms using aqueous mobile phase containing copper (II) ions.},
  author={Ibrahim H. Refaat},
  journal={Bollettino chimico farmaceutico},
  year={2002},
  volume={141 1},
  pages={
          57-62
        }
}
A simple high-performance liquid chromatographic method was developed and validated for the determination of three alpha-amino acids (glycine, lysine, cysteine) and the amino acid derivative (N-acetylhomocysteine thiolactone) using aqueous mobile phase containing copper (II) ions. These amino acids were extracted from tablets, vials, effervescent salts and syrups and quantitatively diluted by the mobile phase. The separation was carried out on zorbax TMS column. The mobile phase was either 1.0… CONTINUE READING