High-performance liquid chromatographic determination of 3 alpha,5 beta-tetrahydroaldosterone in human urine with chemiluminescence detection.

@article{Ishida1992HighperformanceLC,
  title={High-performance liquid chromatographic determination of 3 alpha,5 beta-tetrahydroaldosterone in human urine with chemiluminescence detection.},
  author={Junichi Ishida and Shino Sonezaki and Masatoshi Yamaguchi and Takashi Yoshitake},
  journal={The Analyst},
  year={1992},
  volume={117 11},
  pages={
          1719-24
        }
}
A sensitive method for the determination of 3 alpha,5 beta-tetrahydroaldosterone (THALD) in human urine is described. The method uses high-performance liquid chromatography with chemiluminescence detection. Urinary THALD, released by enzyme hydrolysis, is isolated and concentrated using a Sephadex G-25M column and Bond-Elut C1 cartridges, and then oxidized by copper(II) acetate to form the corresponding glyoxal derivative. The glyoxal derivative is converted into the chemiluminescent… CONTINUE READING