High-performance liquid chromatographic method for the determination of epsilon-(gamma-glutamyl)lysine and mono- and bis-gamma-glutamyl derivatives of putrescine and spermidine.

Abstract

A sensitive, simple, and rapid high-performance liquid chromatographic (HPLC) method is reported for the determination of epsilon-(gamma-glutamyl)lysine and certain gamma-glutamylpolyamines in selected fractions from ion-exchange chromatograms of protein digests. The method involves pre-column derivatization of the gamma-glutamylamine conjugates with o… (More)

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