High-performance liquid chromatographic assay for antiinflammatory agents diclofenac and flurbiprofen in ocular fluids.


An HPLC assay for diclofenac (DC) and flurbiprofen (FP) in a 100-microliters sample of aqueous humour is presented. After acetonitrile extraction, the residue is analyzed using a reversed-phase octyl column and ultraviolet detection. The method is simple and reproducible. Excellent selectivity and resolution are achieved using an acetic acid-acetonitrile… (More)


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