High-performance frontal analysis-high-performance liquid chromatographic system for stereoselective determination of unbound ketoprofen enantiomers in plasma after direct sample injection.

@article{Shibukawa1992HighperformanceFA,
  title={High-performance frontal analysis-high-performance liquid chromatographic system for stereoselective determination of unbound ketoprofen enantiomers in plasma after direct sample injection.},
  author={Akimasa Shibukawa and Akihisa Terakita and Jie He and Terumichi Nakagawa},
  journal={Journal of pharmaceutical sciences},
  year={1992},
  volume={81 7},
  pages={
          710-5
        }
}
An on-line, high-performance frontal analysis (HPFA)-high-performance liquid chromatographic system was developed for the enantioselective determination of a low level of unbound ketoprofen (KP) that is in equilibrium with KP that is bound to protein. The system consists of three subsystems (HPFA system, preconcentration system, and chiral separation system) connected in series in the stated order via column-switching valves. When either a 300-microL portion of buffer solution containing 300 or… CONTINUE READING
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