High-performance closed-tube PCR based on switchable luminescence probes.

Abstract

We introduce a switchable lanthanide luminescence reporter technology based closed-tube PCR for the detection of specific target DNA sequence. In the switchable lanthanide chelate complementation based reporter technology hybridization of two nonfluorescent oligonucleotide probes to the adjacent positions of the complementary strand leads to the formation… (More)
DOI: 10.1016/j.aca.2012.04.027

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