High molecular weight aggregate content of heated and unheated factor VIII products determined by fast-protein liquid chromatography.

Abstract

The molecular-weight distribution of proteins in factor VIII concentrates has been analysed by fast-protein liquid chromatography. The proportion of high-molecular-weight (HMW) aggregates in one product increased on freeze-drying and heating, with fibrinogen and fibronectin being the main protein components of the HMW peak. In all other concentrates, the HMW peak was less than or equal to 5% of the total protein content and there were no differences in HMW content according to purity or method of viral inactivation.

Cite this paper

@article{Dawes1990HighMW, title={High molecular weight aggregate content of heated and unheated factor VIII products determined by fast-protein liquid chromatography.}, author={J. Joan Dawes and Leonard M. Freeman and Noel J. Dawson and Duncan S Pepper and Trevor W. Barrowcliffe}, journal={Vox sanguinis}, year={1990}, volume={58 1}, pages={30-4} }