High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR.

@article{Piatak1993HighLO,
  title={High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR.},
  author={Michael Piatak and Michael S. Saag and L Yang and Stephen J. Clark and John C Kappes and Kin-chun Luk and Beatrice H. Hahn and George M. Shaw and Jeffrey D. Lifson},
  journal={Science},
  year={1993},
  volume={259 5102},
  pages={
          1749-54
        }
}
Quantitative competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated human immunodeficiency virus type-1 (HIV-1) RNA in plasma from 66 patients with Centers for Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from 100 to nearly 22,000,000 copies per milliliter of plasma (corresponding to 50 to 11,000,000 virions per milliliter), was readily quantified in all subjects, was significantly associated with disease stage and CD4+ T cell counts, and… 

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References

SHOWING 1-10 OF 56 REFERENCES

Transient high levels of viremia in patients with primary human immunodeficiency virus type 1 infection.

TLDR
The rapid and spontaneous decline in the viral load suggests an effective immune response in the host that, if understood, may be used to combat AIDS.

Quantitation of plasma human immunodeficiency virus type 1 RNA by competitive polymerase chain reaction.

TLDR
This method offers a sensitive assay with a broad dynamic range for monitoring HIV-1 activity in the plasma of AIDS patients and may provide a useful tool for assessing the effects of antiretroviral therapy.

Quantitation of human immunodeficiency virus type 1 in the blood of infected persons.

TLDR
The levels of HIV-1 in plasma and PBMC are much higher than previous estimates, raising the possibility that the direct cytopathic effect of this retrovirus alone may be sufficient to explain much of the pathogenesis of AIDS.

Plasma viremia in human immunodeficiency virus infection.

TLDR
It is concluded that plasma viremia is a more sensitive virologic marker of the clinical stage of HIV infection and viral replication than the presence of p24 antigen or antibody in plasma.

High titers of cytopathic virus in plasma of patients with symptomatic primary HIV-1 infection.

TLDR
Primary, symptomatic HIV-1 infection is associated with high titers of cytopathic, replication-competent viral strains, and during such infection potential infectivity is enhanced.

HIV‐1 viral DNA load in peripheral blood mononuclear cells from seroconverters and long‐term infected individuals

TLDR
HIV-1 copy numbers were determined using a quantitative polymerase chain reaction (PCR), the PCR-aided template titration assay (PATTY), which utilizes an internal plasmid control DNA, which is amplified within the same tube and using the same primers as the PBMC target DNA.

Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction

TLDR
A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized and proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA.

Exposure to Human Immunodeficiency Virus Type I-Specific T Helper Cell Responses before Detection of Infection by Polymerase Chain Reaction and Serum Antibodies

TLDR
Results, based on the longitudinal study of one individual, showed that the Th cell tests can reveal exposure to HIV-1 antigens several months before evidence of viral infection is detected, even by PCR.

Viral DNA and mRNA expression correlate with the stage of human immunodeficiency virus (HIV) type 1 infection in humans: evidence for viral replication in all stages of HIV disease

TLDR
The data show that the natural history of HIV infection is associated with a shift in the balance of viral expression favoring the production of genomic RNA without a preceding period of true viral latency.
...