High-level transgene expression in neurons by lentivirus with Tet-Off system

@article{Hioki2009HighlevelTE,
  title={High-level transgene expression in neurons by lentivirus with Tet-Off system},
  author={Hiroyuki Hioki and Eriko Kuramoto and Michiteru Konno and Hiroshi Kameda and Takeshi Kaneko},
  journal={Neuroscience Research},
  year={2009},
  volume={63},
  pages={149-154}
}
We developed novel lentiviral vectors by using "Tet-Off system" and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were… CONTINUE READING
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