High-level transgene expression in neurons by lentivirus with Tet-Off system.

@article{Hioki2009HighlevelTE,
  title={High-level transgene expression in neurons by lentivirus with Tet-Off system.},
  author={Hiroyuki Hioki and Eriko Kuramoto and Michiteru Konno and Hiroshi Kameda and Yasuhiro Takahashi and Takashi Nakano and Kouichi C Nakamura and Takeshi Kaneko},
  journal={Neuroscience research},
  year={2009},
  volume={63 2},
  pages={149-54}
}
We developed novel lentiviral vectors by using "Tet-Off system" and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were… CONTINUE READING